How to use detect ractopamine
Ractopamine is a synthetic β adrenergic receptor agonists for the treatment of congestive heart disease, muscular dystrophy, muscle growth, reduce fat accumulation, and fetal and neonatal growth and beneficial. Ractopamine is being used by some as a new pig farms using clenbuterol. This drug in the aquaculture industry scope and safety regulations are not the same around the world. gwunoekmollpoik
In addition, because ractopamine decomposed fat and protein assimilation, ractopamine can make smooth muscle relaxation, ractopamine as asthma in clinical medicine. Therefore, use of the banned ractopamine, its residues may bring harm to consumers. Therefore, the prohibition of adding nutritious foods ractopamine.
Detection is based on the antigen-antibody reaction, microplate coated with capture antibody against the antibody of ractopamine. Join ractopamine antibody, it will connect with the capture antibody. After incubation and washer step after adding standard, the sample solution and ractopamine enzyme markers ( enzyme conjugate ) , free ractopamine and ractopamine and ractopamine competitive enzyme marker antibody ( competitive enzyme immunoassay ), no ractopamine connected enzyme markers are removed in the washing step. The substrate / color agent is added to the wells and incubated. Bound enzyme markers coloring agent into a blue product. After adding stop solution changes the color from blue to yellow. Measured at 450 nm, the absorption intensity in the sample is inversely proportional to the concentration of ractopamine.
Indirect competitive RA elisa kit method using a sample of ractopamine in pre-coated porous strip on the coupling antigen in the sample will ractopamine and microporous strip coupling antigens pre-coated anti- competitive ractopamine antibody, enzyme was added markers, the use of TMB chromogenic substrate, the sample absorbance value contained ractopamine its content has a negative correlation with the standard curve can be derived samples residues of ractopamine. Mouse Insulin ELISA Kit , INS ELISA Kit